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| Registros recuperados: 230 | |
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| Beltrão,Nilzane; Furian,Thales Quedi; Souza,Guilherme Fonseca de; Macagnan,Marisa; Fallavena,Luiz César Bello; Canal,Claudio Wageck. |
| Infectious laryngotracheitis virus (ILTV) cause mild to severe respiratory disease in chickens, the purpose of our study being to use Brazilian isolate of ILTV to reproduce ILTV disease in chickens by experimental infection and to compare three diagnostic methods (nested polymerase chain reaction (PCR), virus isolation, histopathology) for detection of ILTV. Forty-eight chickens intratracheally inoculated with ILTV and a further 48 with PBS, showing mild respiratory signs 48 hours post infection (PI) but no signs of infection after day 10 PI. Every 2 days PI, six birds were arbitrarily selected from the control and infected groups, sacrificed and the trachea collected. Both the nested PCR and virus isolation detected the virus from day 2 until day 12 PI.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Infectious laryngotracheitis virus; Avian pathology; Diagnosis. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500024 |
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| Carvalho,CMC; Litz,FH; Fernandes,EA; Silveira,MM; Martins,JM da S; Fonseca,LA; Zanardo,JA. |
| The aim of this study was to evaluate the incidence of footpad dermatitis and quality of broilers litter fed with sorghum grain and diets based in corn. It was used 544 male and female chicks, distributed in a completely randomized design with two treatments and eight replications. The chicken feed was formulated and produced from corn, soybean meal and sorghum grain. In the formulation were kept constant levels of energy and protein in accordance with the following treatments: A. Control (diet based on corn and soybean meal); B. Grain sorghum (Whole Sorghum + soybean meal). At 35 and 42 days were evaluated mineral matter (A %), calcium (Ca%), phosphorus (P%), pH and dry matter (DM %) of the poultry litter. To evaluate the footpad dermatitis were evaluated... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Turkey; Enteritis; Viruses; Poults; Diagnosis; Calcium; Environmental impact; Phosphorus. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2014000300010 |
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| Thekisoe, Oriel M.M.; Rambritch, Natasha E.; Nakao, Ryo; Bazie, Raoul S.; Mbati, Peter; Namangala, Boniface; Malele, Imna; Skilton, Robert A.; Jongejan, Frans; Sugimoto, Chihiro; Kawazu, Shin-Ichiro; Inoue, Noboru. |
| We have developed two loop-mediated isothermal amplification (LAMP) assays for the detection of Theileria parva, the causative agent of East Coast fever (ECF), an economically important cattle disease in eastern, central and southern Africa. These assays target the polymorphic immunodominant molecule (PIM) and p150 LAMP genes. The primer set for each gene target consists of six primers, and each set recognises eight distinct regions on the target gene to give highly specific detection of T. parva. The detection limit of each primer set is 1 fg, which is equivalent to one copy of the PIM and p150 T. parva genes. These PIM and p150 LAMP primer sets amplify DNA of T. parva isolates from cattle and buffalo from different countries including Kenya, South... |
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Palavras-chave: Theileria parva; LAMP; Buffalo; Cattle; Diagnosis. |
| Ano: 2010 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2672 |
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| FERNANDEZ, M. R.. |
| Objetivos da fitopatologia. Manutenção básica do laboratório. Segurança no laboratório. Esterilização - exigência básica para o trabalho com microorganismos. Estabelecimento de condições assépticas. Métodos de esterilização. Tipos de meios de cultura. Preparo de meios de cultura. Preparo de culturas de fungos e de bactérias. Condições para crescimento de patógenos. Fungos. Bactérias. Incubadoras. Isolamento de fungos e de bactérias de tecido vegetal infectado. Aspectos gerais. Métodos de isolamento. Isolamento de patógenos do solo. Descontaminação de culturas. Aspectos gerais. Métodos mecânicos. Métodos químicos. Ácaros. Isolamento monospórico. Postulados de Koch ou regras de prova da patogenicidade. Técnicas para a inoculação de plantas. Preparo do... |
| Tipo: Documentos (INFOTECA-E) |
Palavras-chave: Diagnosis; Plant disease; Técnica de laboratório.. |
| Ano: 1993 |
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/815639 |
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| Berthe, Franck; Leroux, Frederique; Adlard, Robert; Figueras, Antonio. |
| Among mollusc pathogens, paramyxeans are an important group of protistan parasites belonging to the genera Marteilia and Marteilioides. Martelia refringens and M. sydneyi are of particular concern given their potential impact on mollusc aquaculture world-wide. Aber disease and QX disease are currently listed by the OIE, the World Organisation for Animal Health. After more than thirty years of existence in the scientific literature, these organisms still pose questions and research challenges to investigators. This paper reviews current knowledge of the group and key references. The review was focused on taxonomy, epidemiology, pathology and potential control methods for these organisms in order to enhance understanding of paramyxean infection issues in... |
| Tipo: Text |
Palavras-chave: Paramyxean parasites; Diagnosis; Pathology; Epidemiology; Taxonomy. |
| Ano: 2004 |
URL: https://archimer.ifremer.fr/doc/2004/publication-406.pdf |
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| Costa,E.A.; Lima,G.B.L.; Castro,R.T.; Furtini,R.; Portilho,R.V.; Resende,M.. |
| Um garanhão da raça Brasileiro de Hipismo, de 12 anos de idade, apresentou um quadro neurológico complexo, que se assemelhava, em vários aspectos, aos sinais neurológicos causados pelo herpesvírus eqüino 1 (EHV-1), variando desde uma suave ataxia à completa paralisia dos posteriores. O animal morreu com menos de 12 horas após o início dos sinais nervosos. O diagnóstico de EHV-1 foi feito pela detecção do DNA viral no sistema nervoso central por meio da reação em cadeia da polimerase (PCR) e posterior sequenciamento. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Equine herpesvirus 1; Neurological signs; Clinical case; Diagnosis. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000600044 |
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| SILVA, R. A. M. S.; SANCHEZ, V.; DÁVILA, A. M. R.. |
| A tripanosomose causada pelo Trypanosoma evansi tem uma distribuição geográfica extremamente ampla. Ela ocorre no norte da África, Índia, Malásia, Indonésia, China, Rússia, Filipinas, América Central e América do Sul. O T. evansi causa a tripanosomose equina também conhecida no nordeste da Argentina e Pantanal como "mal de cadeiras". O T. evansi infecta uma ampla variedade de mamíferos e no Pantanal ele tem sido encontrado em cavalos, quoatis (Nasua nasua), cães, capivaras (Hydrochaeris hydrochaeris) e pequenos roedores (Oryzomys sp.). No Pantanal tripanosomose eqüina causa centenas de mortes todo ano. Esta publicação tem como objetivo descrever a metodologia do TIFI utilizada no Laboratório de Sanidade Animal da Embrapa Pantanal. |
| Tipo: Circular Técnica (INFOTECA-E) |
Palavras-chave: Tripanosomose eqüina; Teste de Imunofluorescência Indireta; Trypanosomosis; Diagnosis; Method; Animal sanity.; Diagnostico; Método; Sanidade Animal.. |
| Ano: 2003 |
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/811012 |
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| Silva,Claudênia Ferreira da; Uesugi,Carlos Hidemi; Blum,Luiz Eduardo Bassay; Marques,Abi Soares dos Anjos; Ferreira,Marisa Álvares da Silva Velloso. |
| ABSTRACT: Erwinia psidii causes bacterial blight of guava ( Psidium guajava ), an important disease of this crop in Brazil. The pathogen affects branches and twigs of guava trees, reducing yield significantly. Bacterial dissemination often occurs through contaminated but asymptomatic propagating plant material. The objectives of this research were to evaluate the use of BIO-PCR and conventional PCR to detect E. psidii in inoculated guava plants grown in a greenhouse and in symptomatic and asymptomatic trees from guava orchards. Erwinia psidii strain IBSBF 1576 was inoculated (107CFU mL-1) into young guava shoots and plant tissue was analysed at 0, 5, 10, and 15 days after inoculation. Symptoms were observed after 5 days and all inoculated shoots were PCR... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: BIO-PCR; Diagnosis; Guava bacterial blight; PCR; Psidium guajava. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000901528 |
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| Portella,Luiza Pires; Cadore,Gustavo Cauduro; Sangioni,Luis Antonio; Alves,Marta Elena Machado; Chemeris,Raiza; Brum,Larissa Picada; Vogel,Fernanda Silveira Flores. |
| ABSTRACT: Infections caused by protozoa belonging to the Sarcocystidae family have worldwide distribution and are common in ruminants, leading to considerable economic losses. This study evaluates Sarcocystis spp., Toxoplasma gondii and Neospora caninum infections in sheep from Southwest region of Rio Grande do Sul, Brazil. Myocardium samples of 80 sheep raised on extensive system were collected. Tissue cysts were detected by direct examination and presence of infective agents was confirmed by PCR. Macroscopic evaluation did not reveal changes, but direct microscopic examination showed cysts in 76.2% (61/80, 95% CI: 66.9 - 85.9) samples, and all cysts were morphologically similar to those caused by Sarcocystis tenella or Sarcocystis arieticanis. PCR... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Sarcocystis spp.; Toxoplasma gondii; Neospora caninum; PCR; Diagnosis. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000901613 |
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| Camilo,Lilian Muniz; Pereira-Chioccola,Vera Lucia; Gava,Ricardo; Meira-Strejevitch,Cristina da Silva; Vidal,Jose Ernesto; Mattos,Cinara Cássia Brandão de; Frederico,Fábio Batista; De Mattos,Luiz Carlos; Spegiorin,Lígia Cosentino Junqueira Franco; Murata,Fernando Henrique Antunes; Ferreira,Marina Neves; Barbosa,Deusenia Machado Ulisses; Gonçalves,Fausto da Silva; Dias,Cristiane Moraes; Catelan,Marcia Wakai; Siqueira,Rubens Camargo; Previato,Mariana; Barbosa,Amanda Pires; Cavallini,Danilo. |
| ABSTRACT Symptomatic forms of toxoplasmosis are a serious public health problem and occur in around 10-20% of the infected people. Aiming to improve the molecular diagnosis of symptomatic toxoplasmosis in Brazilian patients, this study evaluated the performance of real time PCR testing two primer sets (B1 and REP-529) in detecting Toxoplasma gondii DNA. The methodology was assayed in 807 clinical samples with known clinical diagnosis, ELISA, and conventional PCR results in a 9-year period. All samples were from patients with clinical suspicion of several features of toxoplasmosis. According to the minimum detection limit curve (in CT), REP-529 had greater sensitivity to detect T. gondii DNA than B1. Both primer sets were retrospectively evaluated using 515... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Toxoplasma gondii; Symptomatic toxoplasmosis; Diagnosis; Real-time polymerase chain reaction. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000600638 |
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| Tavares,RG; Staggemeier,R; Borges,ALP; Rodrigues,MT; Castelan,LA; Vasconcelos,J; Anschau,ME; Spalding,SM. |
| In parasitology, routine laboratory diagnosis involves conventional methods, such as optical microscopy, used for the morphological identification of parasites. Currently, molecular biology techniques are increasingly used to diagnose parasite structures in order to enhance the identification and characterization of parasites. The objective of the present study was to review the main current and new diagnostic techniques for confirmation of parasite infections, namely: polymerase chain reaction (PCR), real-time polymerase chain reaction (RT-PCR), loop-mediated isothermal amplification (LAMP), Luminex xMAP, random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP), and restriction fragment length polymorphism (RFLP), in addition... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Parasite infection; Diagnosis; Molecular techniques; Molecular epidemiology. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000300003 |
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| Caballero,O.L.; Costa,M.C.S.L.; Trevisan,A.; Oliveira,R.M.; Viotti,E.A.; Távora,E.R.F.; Vilaça,S.S.; Sabagga,E.; de-Paula,F.J.; Távora,P.F.; Brasileiro-Filho,G.; Villa,L.L.; Simpson,A.J.G.. |
| To assess the clinical relevance of a semi-quantitative measurement of human cytomegalovirus (HCMV) DNA in renal transplant recipients within the typical clinical context of a developing country where virtually 100% of both receptors and donors are seropositive for this virus, we have undertaken HCMV DNA quantification using a simple, semi-quantitative, limiting dilution polymerase chain reaction (PCR). We evaluated this assay prospectively in 52 renal transplant patients from whom a total of 495 serial blood samples were collected. The samples scored HCMV positive by qualitative PCR had the levels of HCMV DNA determined by end-point dilution-PCR. All patients were HCMV DNA positive during the monitoring period and a diagnosis of symptomatic infection was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Polymerase chain reaction; Human cytomegalovirus; Renal transplant; Diagnosis; Viral load. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001200010 |
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| Registros recuperados: 230 | |
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